Dottorato in Fisiopatologia Medica

Contatti

Supervisore

Benedetta Bussolati

Curriculum vitae

Curriculum Vitae

Tesi di dottorato

Klotho engineered extracellular vesicle in chronic kidney disease preclinical models

Chronic kidney disease, a progressive form of acute kidney injury characterized by renal dysfunction, has a high morbidity and mortality rate related to accelerated aging and cardiovascular disease¹. Extracellular vesicles (EVs) are membranous cargo particles that carry nucleic acids, proteins, organelles, and other biomolecules among cells². Depending on the surface proteins and cargo content, EVs can modulate cell metabolism and the fate of the target cell. In laboratories and clinics, EVs are used in different fields: biomarker discovery, diagnosis, drug delivery and disease therapy. Prof. Bussolati’s group found that urinary EVs carrying Klotho protein recovered kidney function in mouse models with acute tubular injury³. In this study, we aimed to engineer EVs by a Klotho peptide and investigate its potential therapeutic effects in chronic kidney disease preclinical models.

 

^{1.Wang YN, Ma SX, Chen YY, et al. Chronic kidney disease: Biomarker diagnosis to therapeutic targets. Clinica Chimica Acta. 2019;499:54-63. doi:10.1016/j.cca.2019.08.030}

^{2.van Niel G, D’Angelo G, Raposo G. Shedding light on the cell biology of extracellular vesicles. Nat Rev Mol Cell Biol. 2018;19(4):213-228. doi:10.1038/nrm.2017.125}

^{3.Grange C, Papadimitriou E, Dimuccio V, et al. Urinary Extracellular Vesicles Carrying Klotho Improve the Recovery of Renal Function in an Acute Tubular Injury Model. Molecular Therapy. 2020;28(2):490-502. doi:10.1016/j.ymthe.2019.11.013}

Attività di ricerca

Projects

1. (2022-) “Klotho engineered extracellular vesicle in chronic kidney disease preclinical models”, PI: Prof. Benedetta Bussolati
2. (2019-2022) “Investigation of The Effects of SUMO Modification Two Critical Proteins: A Pathogenic NEK1 Mutant That Drives ALS Pathogenesis and The CRISPR-Associated Cas9 Protein”, PI: Assoc. Prof. Umut Şahin

 

Publications

1. Ergunay T., Collino, F.,Bianchi, G. et al. Extracellular vesicles in kidney development and pediatric kidney diseases. Pediatr Nephrol (2023). https://doi.org/10.1007/s00467-023-06165-9
2. Ergünay T., Ayhan, Ö.,Celen, AB. et al. Sumoylation of Cas9 at lysine 848 regulates protein stabilityand DNA binding. Life Sci Alliance (2022). https://doi.org/10.26508/lsa.202101078

 

Attended PhD classes

      2023 (1st year)

1. Fitting regression models: interpretation of regression coefficients, uncertaintymeasures and hypothesis testing. Tutor: Prof. Milena Maule
2. Scientific presentations. Tutor: Prof. Benedetta Bussolati
3. Regression models: from the linear model to the generalised linear models.Interpretation of interactions between predictors. Tutor: Prof. Milena Maule
4. Etiopathogenesis of diabetic retinopathy. Tutor: Prof. Massimo Porta
5. Physiopathology of diabetic nephropathy. Tutor: Assoc. Prof. Gabriella Gruden
6. Update on physiology of hypertension. Tutor: Prof. Franco Veglio
7. Physiopathology of autoimmune hepatitis. Tutor: Assoc. Prof. Marilena Durazzo

 

E-learning training courses

1. General Training Course for Workers on Safety (lec. dec. 81/08), 2022.
2. How to write a scientific paper, 2022.
3. Open science A to Z, 2022.
4. FAIR data, 2022.

 

Attended seminars and courses

1. "Single-cell transcriptomics: cool but is it that simple? & Flow cytometry and single-cellmulti-omics: brothers in arms.", Vladimir Benes, MBC, 09/2023
2. "Introduction to public engagement.", Gabriela Cavaglià and Roberta Pibiri, online formation course, 09/2023
3. "Chemidoc training", Biorad (Ginevra Sacripanti), MBC, 11/2023