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Dott. Paolo Giacomo Arduino

Tesi di dottorato

Salivary extracellular vesicles microRNAs profiling as suitable non-invasive source for biomarkers in oral cancer.

Concise Summary

Several studies investigated the expression of micro RNAs (miRNAs) in saliva as potential biomarkers. Since miRNA associated with extracellular vesicles (EVs) are known to be protected from enzymatic degradation, we firstly evaluated whether salivary EVs of patients with oral squamous cell carcinoma (OSCC) are enriched of selective subsets of miRNAs.

Total RNA was extracted from salivary EVs from OSCC patients and healthy controls. The differential expression of miRNAs was evaluated by microarray and later by qRT-PCR. The discrimination power as biomarkers of miRNAs up-regulated in OSCC patients versus controls was evaluated by the Receiver Operating Characteristic (ROC) curves.

We identified a subset of six miRNAs differentially expressed between OSCC patients and controls. Of these, miR-302b-3p and miR-517b-3p were only found in patients' EVs, while miR-512-3p and miR-412-3p were upregulated in patient's EVs and the ROC curve showed a good discrimination power for OSCC diagnosis; miR-27a-3p and miR-494 were overexpressed in OSCC patients, but the ROC curve showed a lower discrimination power. The Kyoto Encyclopedia of Gene and Genomes pathway analysis showed that the six miRNAs are also suggestively involved in pathway activated in OSCC.

In this present work, for the first time to the best of our knowledge, we confirmed the possibility of using salivary EVs as a non-invasive source of miRNAs for OSCC diagnosis and we identified a subset of miRNAs selectively enriched in EVs of OSCC patients that may be used as possibly biomarkers.



Attività di ricerca


Salivary extracellular vesicle microRNAs profiling as a suitable non-invasive source for biomarkers in patients with oral cancer.

Arduino PG, et al. Possible role for interleukins as biomarkers for mortality and recurrence in oral cancer. Int J Biol Markers 2015;30:e262-6.

Napeñas JJ, Kujan O, Arduino PG, et al. WWOM VI: controversies regarding dental managemnet of medically complex patients: assesment of current recommendations. Oral Surg Oral Med Oral Pathol Oral Radiol 2015;120:207-26.

Gambino A, Carbone M, Arduino PG, et al. Clinical features and histological description of tongue lesions in a large Northern Italian population. Med Oral Patol Oral Cir Bucal 2015;20:e560-5.

Arduino PG, et al. OSCC arising in a patient after hematopoietic stem cell transplanation with BRONJ. Case Rep Dent 2015;2015:831418. 

Arduino PG, et al. Single preoperative dose of prophylactic amoxicillin versus a 2-day postoperative course in dental implant surgery: a two-centre randomised controlled trial. Eur J Oral Implantol 2015;8(2):143-9.

Cafaro A, Arduino PG, et al. Effect of laser acupuncture on salivary flow rate in patients with Sjogren syndrome. Lasers Med Sci 2015;30:1805-9.

Broccoletti R, Cafaro A, Gambino A, Romagnoli E, Arduino PG. Er:Yag Laser versus Cold Knife Excision in the treatment of nondysplastic oral lesions: a randomized comparative study for the postopreative period. Photomed Laser Surg 2015;33:604-9.

Arduino PG, et al. A randomized pilot study to assess the safety and the value of LLLT vs clonazepam in patients with burning mouth syndrome. Lasers Med Sci 2016;31:811-816.

Sindici E, Astesano S, Fazio L, Dragonetti A, Pugliese M, Scully C, Carossa S, Broccoletti R, Arduino PG. Treatment of oral lesions in dystrophic epidermolysis bullosa: a case series of cord blood platelet gel and low-level laser therapy. Acta Derm Venereol. 2017 Mar 10;97(3):383-384.

Arduino PG, et al. A practical clinica recording system for cases with desquamative gingivitis. Br J Dermatol. 2017 Jul;177(1):299-301.

Fung P, et al. Time to onset of bisphosphonate-related osteonecrosis of the jaws: a multicentre retrospective cohort study. Oral Dis. 2017 May;23(4):477-483.

Gambino A, Broccoletti R, Cafaro A, Cabras M, Carcieri P, Arduino PG. Impact of a sodium carbonate spray combined with professional oral hygiene procedures in patients with Sjogren syndrome: an explorative study. Gerodontology. 2017 Jun;34(2):208-214.

Carcieri P, Broccoletti R, Giacometti S, Gambino A, Conrotto D, Cabras M, Arduino PG. Favourably effective formulation of sodium iodide and salicylic acid plus professional hygiene in patients affected by desquamative gingivitis. J Biol Regul Homeost Agents. 2016 Oct-Dec;30(4):1141-1145. 

Arduino PG, Broccoletti R, Carbone M, et al. Describing the gingival involvement in a sample of 182 Italian predominantly oral mucous membrane pemphigoid patients: a retrospective series. Med Oral Patol Oral Cir Bucal. 2017 Mar 1;22(2):e149-e152.

Arduino PG, Romano F, Sasia D, et al. Subgingival microbiota in white patients with desquamative gingivitis: a cross sectional study. J Periodontol. 2017 Jul;88(7):643-650.

Conrotto D, Barattero R, Carbone M, Gambino A, Sciannameo V, Ricceri F, Conrotto F, Broccoletti R, Arduino PG. Can atrophic oral lichen planus promote cardiovascular diseases? A population-based study. Oral Dis. 2017 Jun 19. doi: 10.1111/odi.12700.

Arduino PG. Oral complications of dermatological disorders. Atlas Oral Maxillofac Surg Clin North Am. 2017 Sep;25(2):221-228. 


  • 08-10/10/2015: Bologna (Italia), XIII Congresso S.I.P.M.O. (Società Italiana di Patologie e Medicina Orale)

 Carbone M, Manani G., Conrotto D, Arduino PG, Broccoletti R. Conscious sedation with anxiolytic benzodiazepines in oral surgery according to the Manani protocol: a case series. Sessione Poster.  

 Cafaro A, Gambino A, Broccoletti R, Garrone M, Arduino PG. Placebo versus low level laser therapy in the management of burning mouth syndrome: a randomized controlled single-blind study. Sessione Poster.  

Carcieri P, Broccoletti R, Cabras M, Conrotto D, Arduino PG. Efficacy of professional oral hygiene procedures and "Fertomcidina-u" in patients with plasma cell gingivitis: a case report. Sessione Poster.  

Conrotto D, Giaccone L, Suria M, Broccoletti R, Arduino PG. Reduced salivary flow rate and hematopoietic stem cell transplantation: an emerging problem. Sessione Poster. 

Gambino A, Cafaro A, Arduino PG, Carcieri P, Broccoletti R. Periodontal therapy in patients with primary and secondary Sjogren's syndrome: a double blind randomized clinical trial. Sessione Poster. 

  • 03-05/03/2016: NYC (USA), 1st Oral Global Cancer Forum

Arduino PG, Gai C, Camussi F, Broccoletti R, Giovanni C. Salivary extracellular vesicle microRNA profiling in oral cancer. Preliminary consideration. Sessione Poster.

  • 15-17/09/2016: Turin (Italy), 13° Congresso biennale E.A.O.M. (European Academy of Oral Medicine)

Arduino PG. Maxillary swelling as first manifestation of Burkitt's lymphoma: case report. Comunicazione Orale

Arduino PG. Periodontal status and desquamative gingivitis: clinical and microbiological considerations.Comunicazione Orale.

Cafaro A, Cabras M, Campolongo MG, Gambino A, Valpreda L, Arduino PG. Laser biomodulation and mucous membrane pemphigoid: a randomized split mouth series. Sessione Poster.

Elia A, Carbone M, Conrotto D, Arduino PG, Broccoletti R, Carrozzo M. Mucous membrane pemphigoid: a preliminary analysis of 83 cases. Sessione Poster.

Arduino PG, Gai C, Camussi F, Broccoletti R, Gambino A, Cabras M, Camussi G. A consistent method for RNA extraction from salivary extracellular vesicles. Sessione Poster.

 Conrotto D, Barattero R, D'Aiuto F, Conrotto F, Arduino PG, Broccoletti R. Cardiovascular diseases in different forms of oral lichen planus. Sessione Poster.

Frontiers in regenerative medicine, Turin (ITALY) 18-25 Febbraio 2015 _ PARTECIPANT

D-Day 2015 dottorato di Fisiopatologia Medica, Febbraio 2015, Aula Favretto. AOU Città della Salute e della Scienza_ PARTECIPANT

D-Day 2015 scuole di dottorato, 16 Settembre 2015, Aula Magna Rettorato, Università degli Studi di Torino_ PARTECIPANT

D-Day 2016, 15 settembre 2016, MBC, Torino_ PARTECIPANT

WORKSHOP S.I.P.M.O.Biomarkers ed esami di laboratorio in medicina orale: in quale direzione stiamo andando?, 15 settembre 2016, Centro Congressi Unione Industriale, Torino_ PARTECIPANT/Organizer


Il trasferimento di conoscenze in Ateneo: dalla ricerca alla valorizzazione dell'innovazione, 3 febbraio 2015, Campus Luigi Einaudi, Torino.


17 dicembre 2017: Le Infezioni Da Hpv: Rischi Oncologici e Possibilita' Di Prevenzione. (Ordine Medici Torino ECM).

Prof. Pier Giuseppe Pelicci "Regulation of self renewal in cancer stem cells", 03 febbraio 2015, Aula Magna del Rettorato, Torino.

Prof.ssa Maria Concetta Morrone "Sviluppo e plasticità del sistema visivo dell'uomo", 16 settembre 2015, Aula Magna del Rettorato, Torino.

Prof. Antonio Sica "Myeloid cells as therapeutic target in cancer",  16 settembre 2015, Aula Magna del Rettorato, Torino.



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